Ownership Submission
FORM 4
Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).
UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940
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(Print or Type Responses)
1. Name and Address of Reporting Person *
ANASTASI ROBERT E
  2. Issuer Name and Ticker or Trading Symbol
BROOKS AUTOMATION INC [BRKS]
5. Relationship of Reporting Person(s) to Issuer
(Check all applicable)
_____ Director _____ 10% Owner
__X__ Officer (give title below) _____ Other (specify below)
Executive Vice President
(Last)
(First)
(Middle)
C/O BROOKS AUTOMATION, INC., 15 ELIZABETH DRIVE
3. Date of Earliest Transaction (Month/Day/Year)
10/26/2005
(Street)

CHELMSFORD, MA 01824
4. If Amendment, Date Original Filed(Month/Day/Year)
6. Individual or Joint/Group Filing(Check Applicable Line)
_X_ Form filed by One Reporting Person
___ Form filed by More than One Reporting Person
(City)
(State)
(Zip)
Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned
1.Title of Security
(Instr. 3)
2. Transaction Date (Month/Day/Year) 2A. Deemed Execution Date, if any (Month/Day/Year) 3. Transaction Code
(Instr. 8)
4. Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4 and 5)
5. Amount of Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 3 and 4)
6. Ownership Form: Direct (D) or Indirect (I)
(Instr. 4)
7. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V Amount (A) or (D) Price
Common Stock 10/26/2005   A   2,849 A (1) 2,849 I By 401(k) Plan
Common Stock 10/26/2005   A   7,500 A $ 0.01 7,500 D  

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
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Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned
(e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security
(Instr. 3)
2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (Month/Day/Year) 3A. Deemed Execution Date, if any (Month/Day/Year) 4. Transaction Code
(Instr. 8)
5. Number of Derivative Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4, and 5)
6. Date Exercisable and Expiration Date
(Month/Day/Year)
7. Title and Amount of Underlying Securities
(Instr. 3 and 4)
8. Price of Derivative Security
(Instr. 5)
9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 4)
10. Ownership Form of Derivative Security: Direct (D) or Indirect (I)
(Instr. 4)
11. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares
Stock Option (Right to Buy) $ 16.56 10/26/2005   A   16,650     (2) 02/20/2007 Common Stock 16,650 (3) 16,650 D  
Stock Option (Right to Buy) $ 13.52 10/26/2005   A   22,200     (4) 02/14/2006 Common Stock 22,200 (5) 38,850 D  
Stock Option (Right to Buy) $ 20.82 10/26/2005   A   44,400     (6) 02/19/2008 Common Stock 44,400 (7) 83,250 D  
Stock Option (Right to Buy) $ 59.44 10/26/2005   A   11,100     (8) 02/25/2010 Common Stock 11,100 (9) 94,350 D  
Stock Option (Right to Buy) $ 24.99 10/26/2005   A   16,650     (10) 02/21/2011 Common Stock 16,650 (11) 111,000 D  
Stock Option (Right to Buy) $ 18.11 10/26/2005   A   11,100     (12) 02/20/2012 Common Stock 11,100 (13) 122,100 D  
Stock Option (Right to Buy) $ 7.75 10/26/2005   A   16,650     (14) 04/16/2013 Common Stock 16,650 (15) 138,750 D  
Stock Option (Right to Buy) $ 17.34 10/26/2005   A   16,650     (16) 04/28/2014 Common Stock 16,650 (17) 155,400 D  
Stock Option (Right to Buy) $ 12.69 10/26/2005   A   15,000     (18) 10/26/2012 Common Stock 15,000 (19) 170,400 D  

Reporting Owners

Reporting Owner Name / Address Relationships
 Director  10% Owner  Officer  Other
ANASTASI ROBERT E
C/O BROOKS AUTOMATION, INC.
15 ELIZABETH DRIVE
CHELMSFORD, MA 01824
      Executive Vice President  

Signatures

 /s/ Lynda M. Avallone as Attorney-in-Fact for Robert E. Anastasi   10/28/2005
**Signature of Reporting Person Date

Explanation of Responses:

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1) Received in exhange for 2,567 shares of Helix Technology Corporation common stock in connection with the merger of Helix Technology Corporation and a wholly-owned subsidiary of Brooks Automation, Inc. (the "Merger"). On the effective date of the Merger, the closing price of Helix Technology Corporation's common stock was $13.96 per share, and the closing price of Brooks Automation, Inc.'s common stock was $12.69 per share.
(2) Received in the Merger in exchange for a stock option to acquire Helix Technology Corporation common stock granted on February 20, 1997. This option became exercisable with respect to 25% of such shares subject to the option on each anniversary of the date of grant.
(3) Received in the Merger in exchange for a stock option to acquire 15,000 shares of Helix Technology Corporation common stock for $18.3750 per share.
(4) Received in the Merger in exchange for a stock option to acquire Helix Technology Corporation common stock granted on February 14, 1996. This option became exercisable with respect to 25% of such shares subject to the option on each anniversary of the date of grant.
(5) Received in the Merger in exchange for a stock option to acquire 20,000 shares of Helix Technology Corporation common stock for $15.00 per share.
(6) Received in the Merger in exchange for a stock option to acquire Helix Technology Corporation common stock granted on February 19, 1998. This option became exercisable with respect to 25% of such shares subject to the option on each anniversary of the date of grant.
(7) Received in the Merger in exchange for a stock option to acquire 40,000 shares of Helix Technology Corporation common stock for $23.1094 per share.
(8) Received in the Merger in exchange for a stock option to acquire Helix Technology Corporation common stock granted on February 25, 2000. This option became exercisable with respect to 25% of such shares subject to the option on each anniversary of the date of grant.
(9) Received in the Merger in exchange for a stock option to acquire 10,000 shares of Helix Technology Corporation common stock for $65.9688 per share.
(10) Received in the Merger in exchange for a stock option to acquire Helix Technology Corporation common stock granted on February 21, 2001. This option became exercisable with respect to 25% of such shares subject to the option on each anniversary of the date of grant.
(11) Received in the Merger in exchange for a stock option to acquire 15,000 shares of Helix Technology Corporation common stock for $27.7345 per share.
(12) Received in the Merger in exchange for a stock option to acquire Helix Technology Corporation common stock granted on February 20, 2002. This option becomes exercisable with respect to 25% of such shares subject to the option on each anniversary of the date of grant.
(13) Received in the Merger in exchange for a stock option to acquire 10,000 shares of Helix Technology Corporation common stock for $20.10 per share.
(14) Received in the Merger in exchange for a stock option to acquire Helix Technology Corporation common stock granted on April 16, 2003. This option becomes exercisable with respect to 25% of such shares subject to the option on each anniversary of the date of grant.
(15) Received in the Merger in exchange for a stock option to acquire 15,000 shares of Helix Technology Corporation common stock for $8.5950 per share.
(16) Received in the Merger in exchange for a stock option to acquire Helix Technology Corporation common stock granted on April 28, 2004. This option becomes exercisable with respect to 25% of such shares subject to the option on each anniversary of the date of grant.
(17) Received in the Merger in exchange for a stock option to acquire 15,000 shares of Helix Technology Corporation common stock for $19.2450 per share.
(18) This option becomes exercisable wtih respect to 1/16 of the shares subject to the option every three months following the date of grant, such that the option becomes fully exercisable as of 48 months following the date of grant.
(19) No amount was paid upon grant of the option. The reporting person will pay for the shares upon exercise of the option pursuant to the price listed in Column 2.

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