Ownership Submission
FORM 4
Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).
UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940
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(Print or Type Responses)
1. Name and Address of Reporting Person *
Struve Clayton A
  2. Issuer Name and Ticker or Trading Symbol
PRESSURE BIOSCIENCES INC [PBIO]
5. Relationship of Reporting Person(s) to Issuer
(Check all applicable)
_____ Director __X__ 10% Owner
_____ Officer (give title below) _____ Other (specify below)
(Last)
(First)
(Middle)
175 W JACKSON BLVD, SUITE 440
3. Date of Earliest Transaction (Month/Day/Year)
04/10/2012
(Street)

CHICAGO, IL 60604
4. If Amendment, Date Original Filed(Month/Day/Year)
6. Individual or Joint/Group Filing(Check Applicable Line)
___ Form filed by One Reporting Person
_X_ Form filed by More than One Reporting Person
(City)
(State)
(Zip)
Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned
1.Title of Security
(Instr. 3)
2. Transaction Date (Month/Day/Year) 2A. Deemed Execution Date, if any (Month/Day/Year) 3. Transaction Code
(Instr. 8)
4. Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4 and 5)
5. Amount of Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 3 and 4)
6. Ownership Form: Direct (D) or Indirect (I)
(Instr. 4)
7. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V Amount (A) or (D) Price
Common Stock 04/10/2012   P   2,000 A $ 0.55 102,585 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 04/11/2012   P   4,000 A $ 0.55 106,585 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 04/13/2012   P   2,000 A $ 0.6 108,585 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 04/16/2012   P   4,000 A $ 0.53 112,585 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 04/19/2012   P   9,200 A $ 0.4565 121,785 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 04/20/2012   P   2,000 A $ 0.52 123,785 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 04/25/2012   P   8,000 A $ 0.41 131,785 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 04/24/2012   P   2,000 A $ 0.47 133,785 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 05/10/2012   P   4,000 A $ 0.42 137,785 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 05/11/2012   P   2,000 A $ 0.42 139,785 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 05/14/2012   P   2,000 A $ 0.4 141,785 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 06/06/2012   P   150 A $ 0.21 141,935 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 06/18/2012   P   5,000 A $ 0.25 146,935 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 07/23/2012   P   2,000 A $ 0.28 148,935 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 08/28/2012   P   2,000 A $ 0.28 150,935 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 09/04/2012   P   2,000 A $ 0.29 152,935 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 09/10/2012   P   10,000 A $ 0.28 162,935 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 09/18/2012   P   2,000 A $ 0.25 164,935 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 09/28/2012   J(2)   27,816 A $ 0 (3) 1,051,665 D  
Common Stock 10/04/2012   P   10,000 A $ 0.29 174,935 I Reporting Person is a member manager of CCS, LLC (1)
Common Stock 10/08/2012   P   2,000 A $ 0.29 176,935 I Reporting Person is a member manager of CCS, LLC (1)

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
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Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned
(e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security
(Instr. 3)
2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (Month/Day/Year) 3A. Deemed Execution Date, if any (Month/Day/Year) 4. Transaction Code
(Instr. 8)
5. Number of Derivative Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4, and 5)
6. Date Exercisable and Expiration Date
(Month/Day/Year)
7. Title and Amount of Underlying Securities
(Instr. 3 and 4)
8. Price of Derivative Security
(Instr. 5)
9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 4)
10. Ownership Form of Derivative Security: Direct (D) or Indirect (I)
(Instr. 4)
11. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares
Series C Common Stock Purchase Warrant $ 0.74 07/06/2012(4)   J(4)     311,925   (4) 08/07/2017 Common Stock (5) 311,925 (5) (4) 0 D  
Series C Common Stock Purchase Warrant $ 0.6 07/06/2012(4)   J(4)   311,925     (4) 08/07/2017 Common Stock (5) 311,925 (5) (4) 311,925 D  
Promissory Note - Aug 2011 Common Stock Warrant $ 0.85 07/06/2012(6)   J(6)     211,765   (6) 08/03/2014 Common Stock (7) 211,765 (7) (6) 0 D  
Promissory Note - Aug 2011 Common Stock Warrant $ 0.6 07/06/2012(6)   J(6)   211,765     (6) 08/03/2014 Common Stock (7) 211,765 (7) (6) 211,765 D  
Promissory Note - Aug 2011 Common Stock Warrant $ 0.76 07/06/2012(8)   J(8)     26,316   (8) 08/03/2014 Common Stock (7) 26,316 (7) (8) 0 D  
The reported transactions involved an amendment of an outsta $ 0.6 07/06/2012(8)   J(8)   26,316     (8) 08/03/2014 Common Stock (7) 26,316 (7) (8) 23,316 D  
February 2012 Common Stock Purchase Warrant $ 0.74 07/06/2012(4)   J(4)     200,001   (4) 08/07/2012 Common Stock (9) 200,001 (9) (4) 0 D  
February 2012 Common Stock Warrant $ 0.6 07/06/2012(4)   J(4)   200,001     (4) 08/07/2017 Common Stock (9) 200,001 (9) (4) 200,001 D  

Reporting Owners

Reporting Owner Name / Address Relationships
 Director  10% Owner  Officer  Other
Struve Clayton A
175 W JACKSON BLVD
SUITE 440
CHICAGO, IL 60604
    X    
CSS LLC/IL
175 WEST JACKSON BLVD
SUITE 440
CHICAGO, IL 60604
      (a)

Signatures

 Clayton A. Struve   11/20/2012
**Signature of Reporting Person Date

 Mitchell R. Bialek   11/20/2012
**Signature of Reporting Person Date

Explanation of Responses:

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1) Reporting Person, Clayton Struve, is an Indirect Owner as Member Manager of CSS, LLC of shares purchased by CSS, LLC. CSS,LLC disclaims any beneficial ownership (direct or indirect) of shares held directly by Reporting Person, Clayton Struve.
(2) Dividend shares as a result of investment in Series C Preferred shares.
(3) Dividend accrual of $22,322 at a rate of .8025 or 27,816 shares
(4) The reported transactions involved an amendment of an outstanding warrant, resulting in the deemed cancellation of the old warrant at an exercise price of $0.74 and the grant of a replacement warrant at an exercise price of $0.60.
(5) The Series C Common Stock Purchase Warrants contain a limitation on exercise which prevents the Reporting Person from exercising any Warrants if, after giving effect to the exercise, the Reporting Person would beneficially own more than 4.99% of the outstanding shares of Common Stock. The Reporting Person may elect to increase this limitation to 9.99%, 14.99% or 19.99%, upon not less than 61 days prior written notice to the Company. Since the Reporting Person currently beneficially owns more than 9.99% but less than 14.99% of the outstanding shares of Common Stock, the conversion limitation that applies to the Reporting Person is the 14.99% limitation. The Reporting Person disclaims beneficial ownership of such securities except to the extent of the Reporting Person's pecuniary interest in such securities
(6) The reported transactions involved an amendment of an outstanding warrant, resulting in the deemed cancellation of the old warrant at an exercise price of $0.85 and the grant of a replacement warrant at an exercise price of $0.60.
(7) The Promissory Note-Aug 2011 Common Stock Purchase Warrants contain a limitation on exercise which prevents the Reporting Person from exercising any Warrants if, after giving effect to the exercise, the Reporting Person would beneficially own more than 4.99% of the outstanding shares of Common Stock. The Reporting Person may elect to increase this limitation to 9.99%, 14.99% or 19.99%, upon not less than 61 days prior written notice to the Company. Since the Reporting Person currently beneficially owns more than 9.99% but less than 14.99% of the outstanding shares of Common Stock, the conversion limitation that applies to the Reporting Person is the 14.99% limitation. The Reporting Person disclaims beneficial ownership of such securities except to the extent of the Reporting Person's pecuniary interest in such securities
(8) The reported transactions involved an amendment of an outstanding warrant, resulting in the deemed cancellation of the old warrant at an exercise price of $0.76 and the grant of a replacement warrant at an exercise price of $0.60
(9) The February 2012 Common Stock Purchase Warrants contain a limitation on exercise which prevents the Reporting Person from exercising any Warrants if, after giving effect to the exercise, the Reporting Person would beneficially own more than 4.99% of the outstanding shares of Common Stock. The Reporting Person may elect to increase this limitation to 9.99%, 14.99% or 19.99%, upon not less than 61 days prior written notice to the Company. Since the Reporting Person currently beneficially owns more than 9.99% but less than 14.99% of the outstanding shares of Common Stock, the conversion limitation that applies to the Reporting Person is the 14.99% limitation. The Reporting Person disclaims beneficial ownership of such securities except to the extent of the Reporting Person's pecuniary interest in such securities
 
Remarks:
(a) Reporting Person Clayton A. Struve is a member of manager of CSS, LLC and has indirect beneficial ownership of shares of stock held in the name of CSS, LLC.  CSS, LLC disclaims any beneficial ownership (direct or indirect) of shares held directly by Reporting Person, Clayton Struve.

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